Polyclonal anti-mouse IgE antibody

SKU: HE.02.01 Categories: ,

Description

Polyclonal anti-mouse IgE antibody

The affinity purified goat anti-mouse IgE specifically reacts with the IgE heavy chain and not with IgG, IgA, IgM or IgD. It is suitable for use as capture or detection antibody in various immunoassays.

  • Product name: Anti-Mouse IgE antibody
  • Form: Freeze-dried (lyophilized)
  • Size: 133 µg, sufficient for coating one 96-well plate
  • Reactivity: Mouse
  • Host: Goat
  • Antibody type: Secondary antibody
  • Specificity/ target: IgE
  • Clonality: Polyclonal
  • Isotype: IgG
  • Purity: Antigen affinity purified
  • Suitable for: ELISA, Immunoelectrophoresis, Western Blotting
  • Please reconstitute with 1 ml, dilute this 1 ml to 10 ml. Optimal dilutions/ concentrations should be determined by the end user.

Quantification of antigen specific serum antibodies

Together with our biotinylated HDM allergen products, this antibody can be used to quantify HDM allergen specific IgE antibodies in mouse serum using an ELISA method. The antibody can be coated on an ELISA plate and act as a capture antibody to immobilize serum IgE antibodies. In the next step biotinylated HDM allergens are added to facilitate the detection of HDM allergen specific antibodies of the IgE isotype. Using a reference serum or by comparing multiple sera the HDM allergen specific antibodies can be (relatively) quantified.

A perfect way for researchers, who are working on allergic asthma research with mouse models, to determine whether immunizations of mice with house dust mite (HDM) have been successful by looking at the amount of specific antibodies against HDM proteins in the serum of mice. For more information about biotinylation we have a page which describes the protocol for the biotinylation products and antibodies.

Anti-mouse antibodies

  • Tested with HDM immunized serum
  • Freeze-dried (lyophilized) material
    • Long term storage e.g. at – 20°C
    • Every buffer applicable to dissolve the antibodies
    • Does not contain any salts
    • Ready to use in your desired buffer
  • Polyclonal antibodies
    • Broader affinity
  • Part of a group of related antibodies to be used in this application.
  • Reference mouse serum available on request

Antibodies and biotinylation kit

The specificity of the captured antibodies for HDM must be tested with biotinylated HDM or the biotinylated major allergens like biotinylated Der p 1 or biotinylated Der p 2. The antibodies and biotinylated products are available as separate products but are also available in a handy kit which contains the anti-mouse IgE/ anti-mouse IgG1/ anti-mouse IgG2A and biotinylated HDM (or biotinylated Der p 1/ biotinylated Der p 2).

Pricing and orderinformation

Our products are available for worldwide shipping and are sent with FedEx. Orders are shipped within 24 hours after receipt of your order. For more information and pricing please send us an e-mail or give us a call. We will respond as soon as possible.

You can send us your request on our e-mail address info@citeq.com, give us a call +31(0)50-3137464 or fill in our contact form.