Der p 1 ELISA kit
The Der p 1 ELISA kit is used for measuring the major allergen Der p 1. The Dermatophagoides pteronyssinus protein Der p 1 is one of the major house dust mite allergens. Using this Enzyme-Linked Immunosorbent Assay (ELISA) kit the concentration of this allergen in solution can be determined. This kit provides sufficient antibodies and Der p 1 standard to perform ELISA experiments on five 96-Well plates.
The ELISA kit is sufficient for the coating of five 96-Well plates.
Contents of the Der p 1 ELISA kit
- 1 vial with capture antibody (white vial with blue endcap)
- 1 vial with detection antibody (brown vial with brown endcap)
- 5 vials with Der p 1 standard (white vial with wite endcap)
All components of the kit are supplied in freeze-dried (lyophilized) form in 0.5 ml vials. It has a longer shelf life than the regular ELISA kits which will be delivered in liquid form. One of the advantages of our kit is that the Der p 1 standard will be delivered in 5 separate vials, this way you can use 1 vial with the right amount for each plate. Just dissolve the material in your buffer of choice and you are ready to coat the plate.
Standard Der p 1
For an assay a standard is a necessity. Not only for performing the assay but also for comparing the assay to other assays. However, there is no official international standard for Der p 1, yet. Citeq aims to establish a standard with the potential of being the first international standard. The basis for developing an international standard is using well known accepted methods for Der p 1 and one of these methods is weighing.
Using weight as a standard is possible by purifying natural allergens like Der p 1 from Dermatophagoides pteronyssinus to a high degree (> 98%). The purity is then confirmed using well established analytical methods, e.g. SDS PAGE, (CBB and silver staining), protein determination (Bradford and BCA), optical density and mass spectrometry. The mass of these purified allergens (> 98%) form the basis for Citeq’s golden standard. With such an accurate quantification at Citeq’s disposal this standard has the potential to be an international reference standard.
Based on the golden standard we are able to produce a reliable ELISA kit to determine Der p 1 concentrations in Dermatophagoides pteronyssinus extracts.
- This kit is intended for research use only.
- Upon arrival the kit should be stored at -20°C in a non-frost free freezer.
- The kit should not be used beyond its expiry date.
- Wear disposable (latex) gloves when handling specimens and reagents.
- Use disposable pipette tips throughout the procedure to avoid contamination of reagents.
- Click here for the test procedure and further background information for the performance of the ELISA.
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