Antibodies and biotinylation kit


Antibodies and biotinylation kit

Our polyclonal anti-mouse antibodies can be used together with our biotinylated HDM allergen products to quantify HDM allergen specific antibodies in mouse serum using an ELISA method. This kit contains the IgE/ IgG1/ IgG2a antibodies and biotinylated D. pteronyssinus HDM.

Quantification of antigen specific serum antibodies

The antibody can be coated on an ELISA plate and act as a capture antibody to immobilize serum IgE/ IgG1/ IgG2a antibodies. In the next step biotinylated HDM allergens are added to facilitate the detection of HDM allergen specific antibodies of the IgE/ IgG1/ IgG2a isotype. Using a reference serum or by comparing multiple sera the HDM allergen specific antibodies can be (relatively) quantified.

A perfect way for researchers, who are working on allergic asthma research with mouse models, to determine whether immunizations of mice with house dust mite (HDM) have been successful by looking at the amount of specific antibodies against HDM proteins in the serum of mice.

The specificity of the captured antibodies for HDM must be tested with biotinylated HDM or the biotinylated major allergens like biotinylated Der p 1 or biotinylated Der p 2. The antibodies and biotinylated products are available as separate products but are also available in a handy kit which contains the anti-mouse IgE/ anti-mouse IgG1/ anti-mouse IgG2A and biotinylated HDM extract (or biotinylated Der p 1/ biotinylated Der p 2). For more information about biotinylation we have a page which describes the protocol for the biotinylation products and antibodies.

Content of the kit

  • 1 x anti-mouse IgE (sufficient for coating  1 x 96-well plate)
  • 1 x anti-mouse IgG1 (sufficient for coating  1 x 96-well plate)
  • 1 x anti-mouse IgG2A (sufficient for coating  1 x 96-well plate)
  • You can choose from the following biotinylated products:
    • Biotinylated D. pteronyssinus (HDM) extract
    • Biotinylated Der p 1
    • Biotinylated Der p 2

This kit does not include the standard and reference mouse serum. Both can be delivered on request, please contact us for more information.

Pricing and orderinformation

Our products are available for worldwide shipping and are sent with FedEx. Orders are shipped within 24 hours after receipt of your order. For more information and pricing please send us an e-mail or give us a call. We will respond as soon as possible.

You can send us your request on our e-mail address, give us a call +31(0)50-3137464 or fill in our contact form.