Induced asthma – Acute challenge model (21 days)

Model 4: Acute challenge model (21 days)

  • Description: Challenged with HDM by intranasal administration on days 2, 4, 6, 9, 11, 13, 16,18,20. Mice were sacrificed 24 hours after the last challenge.
  • Duration: 21 days
  • Attributes: Acute challenge model
  • Endpoints: Airway hyper-reactivity and detailed lung mechanics; flexiVent™. Histopathology and immunohistochemistry. Total and differential cell count in the BALF/Lung.
  • Material used: D. pteronyssinus extract from Citeq

Lung function:

Increase in airway resistance and decrease in airway compliance is equal across different batches of Citeq HDM extracts.

H&E staining:

HDM extract causes inflammation of the airway

Differential cells analysed by flow cytometry:

Botch batches of Citeq induce a response in alveolar macrophage (AM), eosinophil (E) and neutrophil (N) cells. Batch 15J01 induces a larger response in comparison to batch 15G10.

All allergic asthma models

  1. Acute inflammatory model (7 days)
  2. Acute sensitization/ challenge model (14 days)
  3. Acute sensitization/ challenge model (17 days)
  4. Acute challenge model (21 days)
  5. New HDM (immuno)therapy testing (45 days)
  6. Sensitization/ challenge model (60 days)

Our HDM extracts