Frequently Asked Questions
Often we receive questions from our customers. Therefore, we decided to answer the most common questions here.
If you still do not manage to find an answer to your question, please do not hesitate to contact us.
How should allergens be stored?
How should I store the products after I dissolved them with a PBS buffer?
How can I order your products?
At what temperature do you ship your products
What is the difference between a whole culture and mite bodies?
Freeze dried mite bodies (.30) consists only, at least for 95%, of mite bodies (ratio Der p 1/Der p 2 is 1:1). Downstream processing of mite bodies results in a freeze dried product which has higher Der p 1 and Der p 2 concentrations than mite bodies (.30).
In conclusion, it is simply a question of quantities and ratios of Der p 1/Der p 2 in Whole cultures (.60) and mite bodies (.30).
I am using your biotinylated HDM products. Do you have a protocol for the use of these biotinylated products?
What kind of medium do you use in producing your mite cultures which give the mite allergens?
Can I re-order material from a specific LOT/ batch?
Can you deliver excrements of the house dust mites?
Why is it important to work with purified allergens rather than allergen extracts?
What is the difference between the forms you offer?
.30: Mite bodies: purified mite bodies, this does not contain other material such as the faeces of the mites. Delivered in total dry weight.
.40: Semi-purified mite bodies: semi-purified mite bodies. Less purified then the .30. Delivered in total dry weight.
.60: Whole culture: A whole culture consist of all the material of the house dust mite cultivation. This means: mite bodies, eggs, larves, cultivation medium and the faeces. Delivered in total dry weight.
.64: Extract with low level of endotoxin: the same as our regular .85 extract but with a low level of endotoxin. Delivered in total dry weight. We only have this product available of the D. pteronyssinus mite.
.71: purified Group 1 allergen (Der p 1 or Der f 1). Based on protein content (BCA).
.72: purified Group 2 allergen (Der p 2 or Der f 2). Based on protein content (BCA).
.85: Extract (of whole culture): the whole culture which is extracted. A whole culture consist of all the materials of the cultivation, including bodies, eggs, larvae, skin parts, cultivation medium and the excrements. This is the regular extract which is the most used by researchers. Delivered in total dry weight.
.86: Extract (of mite bodies): purified mite bodies which is extracted, this does not contain other material such as the faeces of the mites. Delivered in total dry weight.
.87: Excrements: the purified excrements (faeces/ droppings) of the house dust mites. We have excrement extracts available from D. farinae and D. pteronyssinus. Delivered in total dry weight.
.95: Extract: Regular HDM extract but not filled out on dry weight but on protein content (BCA). These are small vials which are developed for researchers.
In what form do you deliver your products?
How long can I store your products?
Do you ship the products worldwide?
Can we use your raw materials as an active pharmaceutical ingredient (API) starting materials?
I am working with animal models. Do you have examples of models in which house dust mites (HDMs) are used?
What buffer should I use to dissolve the products?
What production process do you have for your moulds?
Are your products suitable for in-humans use and immunotherapy?
How are allergens purified?
How to dissolve mould extracts
Are the products dialysed before freeze-drying?
Our products will be delivered in freeze-dried (lyophilised) form. Before freeze drying the products are dialysed in a volatile buffer. This results in salt free end product. You can dissolve the product in a buffer of your choice.
HDM ELISA kits With the release of our house dust mite ELISA kits we have extended our HDM product catalogus. For the house dust mite D. pteronyssinus we have the Der p 1 ELISA kit and Der p 2 ELISA kit available. For the house dust mite D. farinae we have the Der f 1...
Effective SCIT using purified allergens in a murine asthma model Does the use of purified major house dust mite allergens (Der p 1 & Der p 2) in a murine asthma model achieve superior suppression of allergic manifestations compared to an allergen extract? This is the...
Introduction On this page we describe the results for determining the solubility of HDM extracts. We have dissolved the Dermatophagoides pteronyssinus extract FD (freeze-dried) and whole culture D. farinae extract FD (freeze-dried) in 70% DMSO. We have compared this...