Effective SCIT using purified allergens in a murine asthma model
Does the use of purified major house dust mite allergens (Der p 1 & Der p 2) in a murine asthma model achieve superior suppression of allergic manifestations compared to an allergen extract? This is the main topic in a new scientific publication from Laura Hesse of the University Medical Center Groningen (UMCG) in the Netherlands. The full article can be read in the European Journal of Allergy and Clinical Immunology. On this page an abstract of the article.
Allergen extracts or purified natural allergens
Allergen-specific immunotherapy can induce long-term suppression of allergic symptoms, reduce medication use and prevent exacerbations of allergic rhinitis and asthma. Current treatment is based on crude allergen extracts, which contain immunostimulatory components such as β-glucans, chitins and endotoxin. Use of purified or recombinant allergens might therefore increase efficacy of treatment. Application of purified natural group 1 and 2 allergens from Dermatophagoides pteronyssinus (Der p) for subcutaneous immunotherapy (SCIT) treatment was tested in a house dust mite (HDM) driven mouse model of allergic asthma.
HDM-sensitized mice received SCIT with HDM extract, a mixture of Der p 1 and 2 (DerP1/2), or placebo. Upon challenges, we measured specific immunoglobulin responses, allergen-induced ear swelling (ESR), airway hyper responsiveness (AHR) and inflammation in broncho-alveolar lavage fluid (BAL) and lung tissue.
ESR measurement shows suppression of early allergic response in HDM- and DerP1/2-SCIT treated mice. Both HDM-SCIT and DerP1/2-SCIT are able to suppress AHR and eosinophilic airway inflammation. In contrast, only DerP1/2-SCIT is able to significantly suppress type-2 cytokines in lung tissue and BAL fluid. Moreover, DerP1/2-SCIT treatment is uniquely able suppress CCL20 and showed a trend towards suppression of IL-33, CCL17 and eotaxin levels in lung tissue.
Major allergens increase clinical efficacy of SCIT
Taken together, these data show that SCIT with Der p 1/ Der p 2 is able to not only suppress AHR and inflammation, but also has superior activity towards suppression of Th2 cells and HDM-induced activation lung structural cells including the airway epithelium. Postulation is that treatment with purified natural major allergens derived from HDM will likely increase clinical efficacy of SCIT.
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Used allergens in this publication
The allergens which were used for this research can be purchased from Citeq Biologics. Contact us for pricing and availability.