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Development of a enzyme-linked immunosorbent assay kit for determination of the major allergen from Dermatophagoides pteronyssinus, Der p1, house dust mite

June 9, 2011

Dermatophagoides pteronyssinus 1 (Der p1) is a major mite allergen in Dermatophagoides pteronyssinus house dust mite. Currently there are no reliable ready-to-use-kits for the determination of Der p1. Therefore we developed a ready-to-use-ELISA for Der p1.
A ninety-six-wells plate (NUNC Maxisorp) was coated with a monoclonal antibody (clone 9-5-1) against Der p1 generated in a mouse…. Read more »

Enzymatic Characterization of House Dust and Storage Mite Allergen Extracts

May 18, 2010

There is scarce information about the biological function of many allergens in house dust and storage mites. In the last years, several studies have reported on the enzymatic activity of some mite allergens. To investigate the enzymatic activity in whole cultures and mite bodies of house dust and storage mites, chitinase, collagenase, cysteine protease and… Read more »